Description
TopTaq DNA Polymerase contains three binding proteins: one binding protein binds to double-stranded DNA template, preventing polymerase activity at room temperature, and the other two binding proteins bind primers, preventing primer-dimer formation. Together, these binding proteins reduce non-specific amplification. The blocking proteins are released from primers and templates during the initial denaturation. This double-blocking method has higher efficiency and specificity than antibody-based or chemically-modified hot start PCR. Template-independent ""A"" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into T-vectors. This kit does not use Taq antibodies, reducing the risk of mammalian DNA contamination, and this kit does not use chemical modification, so a long denaturing step is not required. Genomic DNA fragments can be amplified up to 15 kb. This kit does not contain 2.5 mM dNTPs